Primers Available
Organism:Alosa sapidissima mitochondrion
Repeat:(TCC)4
Start:5805 End:5816
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGACTGGTCCCCCTTATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGACTGTTCACCCGGTTCC | |||||||||||||
| Tm_F_1 | 60.309 | Size_F_1 | 20 | Tm_R_1 | 59.966 | Size_R_1 | 20 | PRODUCT Size | 147 bp | Primer Start | 5718 | Primer End | 5864 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCACGAATGAACAACATGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAGACTGTTCACCCGGTTCC | |||||||||||||
| Tm_F_2 | 60.367 | Size_F_2 | 20 | Tm_R_2 | 59.966 | Size_R_2 | 20 | PRODUCT Size | 105 bp | Primer Start | 5760 | Primer End | 5864 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGACTGGTCCCCCTTATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAATGGCCCCAAGAATTGA | |||||||||||||
| Tm_F_3 | 60.309 | Size_F_3 | 20 | Tm_R_3 | 60.26 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 5718 | Primer End | 5966 |