Primers Available
Organism:Alosa sapidissima mitochondrion
Repeat:(CTTA)3
Start:4499 End:4510
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAATCACCGGACAATGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTTAAGTCCCCCTCACCCTC | |||||||||||||
| Tm_F_1 | 60.048 | Size_F_1 | 20 | Tm_R_1 | 59.795 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 4281 | Primer End | 4559 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAAGGAATCACGCTAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTAAGTCCCCCTCACCCTC | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.795 | Size_R_2 | 20 | PRODUCT Size | 158 bp | Primer Start | 4402 | Primer End | 4559 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAATCACCCAACTCTCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTAAGTCCCCCTCACCCTC | |||||||||||||
| Tm_F_3 | 59.903 | Size_F_3 | 20 | Tm_R_3 | 59.795 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 4299 | Primer End | 4559 |