Primers Available
Organism:Megacrania alpheus adan mitochondrion
Repeat:(AATA)5
Start:7899 End:7918
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCCGAACATATAATCCCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTTATTCTTTGATGTTATTCTTTGTG | |||||||||||||
| Tm_F_1 | 59.52 | Size_F_1 | 21 | Tm_R_1 | 58.611 | Size_R_1 | 27 | PRODUCT Size | 258 bp | Primer Start | 7695 | Primer End | 7952 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCCGAACATATAATCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTATTCTTTGATGTTATTCTTTGTG | |||||||||||||
| Tm_F_2 | 58.2 | Size_F_2 | 20 | Tm_R_2 | 58.611 | Size_R_2 | 27 | PRODUCT Size | 258 bp | Primer Start | 7695 | Primer End | 7952 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGATTAATAAACAAATCCCCGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTATTCTTTGATGTTATTCTTTGTG | |||||||||||||
| Tm_F_3 | 60.037 | Size_F_3 | 22 | Tm_R_3 | 58.611 | Size_R_3 | 27 | PRODUCT Size | 273 bp | Primer Start | 7680 | Primer End | 7952 |