Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(T)12
Start:27020 End:27031
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGATCCGCAGCTGACAATA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTTGTCTTTCCTGACCGA | |||||||||||||
| Tm_F_1 | 59.972 | Size_F_1 | 20 | Tm_R_1 | 58.325 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 26789 | Primer End | 27055 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTCGACCAATCCTTCCTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTTGTCTTTCCTGACCGA | |||||||||||||
| Tm_F_2 | 60.066 | Size_F_2 | 20 | Tm_R_2 | 58.325 | Size_R_2 | 20 | PRODUCT Size | 182 bp | Primer Start | 26874 | Primer End | 27055 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTCCAATCAACTGATCCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTTGTCTTTCCTGACCGA | |||||||||||||
| Tm_F_3 | 59.9 | Size_F_3 | 20 | Tm_R_3 | 58.325 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 26778 | Primer End | 27055 |