Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(T)10aagt(A)11
Start:17024 End:17048
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACTCCTGCTTCCAGCATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCCCGGTAGAAGAGAAGGT | |||||||||||||
| Tm_F_1 | 59.957 | Size_F_1 | 20 | Tm_R_1 | 60.067 | Size_R_1 | 20 | PRODUCT Size | 161 bp | Primer Start | 16946 | Primer End | 17106 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACTCCTGCTTCCAGCATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGTGGATAAACGACCAATCC | |||||||||||||
| Tm_F_2 | 59.957 | Size_F_2 | 20 | Tm_R_2 | 60.192 | Size_R_2 | 20 | PRODUCT Size | 178 bp | Primer Start | 16946 | Primer End | 17123 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGCTTCCAGCATCTCTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGGATAAACGACCAATCC | |||||||||||||
| Tm_F_3 | 60.096 | Size_F_3 | 20 | Tm_R_3 | 60.192 | Size_R_3 | 20 | PRODUCT Size | 172 bp | Primer Start | 16952 | Primer End | 17123 |