Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(T)10
Start:1780 End:1789
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCGTGTCAACCAATATCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCGACTAGTTCCGGGTTC | |||||||||||||
| Tm_F_1 | 59.247 | Size_F_1 | 20 | Tm_R_1 | 60.328 | Size_R_1 | 20 | PRODUCT Size | 224 bp | Primer Start | 1718 | Primer End | 1941 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGTGTCAACCAATATCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGGCAACCCATTATGATTA | |||||||||||||
| Tm_F_2 | 59.247 | Size_F_2 | 20 | Tm_R_2 | 60.528 | Size_R_2 | 20 | PRODUCT Size | 198 bp | Primer Start | 1718 | Primer End | 1915 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCGTGTCAACCAATATCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCCGACTAGTTCCGGGTTC | |||||||||||||
| Tm_F_3 | 60.103 | Size_F_3 | 21 | Tm_R_3 | 60.328 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 1717 | Primer End | 1941 |