Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(AT)6
Start:65622 End:65633
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTCTTTCGGGCAGTTATCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCACCTCATTCGTTGACTT | |||||||||||||
| Tm_F_1 | 59.708 | Size_F_1 | 20 | Tm_R_1 | 59.966 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 65553 | Primer End | 65822 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTCTTTCGGGCAGTTATCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCCACCTCATTCGTTGAC | |||||||||||||
| Tm_F_2 | 59.708 | Size_F_2 | 20 | Tm_R_2 | 59.827 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 65553 | Primer End | 65824 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTCGGGCAGTTATCGAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAAGGTAGGGGCCCATGAAT | |||||||||||||
| Tm_F_3 | 60.202 | Size_F_3 | 20 | Tm_R_3 | 60.522 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 65557 | Primer End | 65763 |