Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(A)13
Start:5715 End:5727
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GATCCATAAACCCATCAAATCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAATGATCCAAGGGAAAGA | |||||||||||||
| Tm_F_1 | 59.908 | Size_F_1 | 23 | Tm_R_1 | 59.864 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 5664 | Primer End | 5943 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCATAAACCCATCAAATCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAATGATCCAAGGGAAAGA | |||||||||||||
| Tm_F_2 | 57.804 | Size_F_2 | 21 | Tm_R_2 | 59.864 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 5666 | Primer End | 5943 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCGTACGGCTCGAGAAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAGGGGTTAGAGACCACTCAA | |||||||||||||
| Tm_F_3 | 60.514 | Size_F_3 | 20 | Tm_R_3 | 59.144 | Size_R_3 | 22 | PRODUCT Size | 238 bp | Primer Start | 5591 | Primer End | 5828 |