Primers Available
Organism:Theobroma cacao chloroplast
Repeat:(A)10
Start:136180 End:136189
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCCACTGGATCTGTTCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGCGGAAAAATAGCTCGAC | |||||||||||||
| Tm_F_1 | 60.048 | Size_F_1 | 20 | Tm_R_1 | 59.982 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 136148 | Primer End | 136423 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCACTGGATCTGTTCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAAAATAGCTCGACGCCAG | |||||||||||||
| Tm_F_2 | 60.048 | Size_F_2 | 20 | Tm_R_2 | 59.982 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 136148 | Primer End | 136418 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCATGATTTTCCTACCCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCTTAACCCGAAATGGCTG | |||||||||||||
| Tm_F_3 | 60.126 | Size_F_3 | 20 | Tm_R_3 | 60.067 | Size_R_3 | 20 | PRODUCT Size | 211 bp | Primer Start | 136015 | Primer End | 136225 |