Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)14atttgctctttcactgcgaaggggctaagtaaaaaaagaaatattgtttgtccaaaac(A)10gaaacctataattttg(T)11
Start:87120 End:87228
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGCTGTTATCCGCTACATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGCAATTCATTATTTCGGG | |||||||||||||
| Tm_F_1 | 60.117 | Size_F_1 | 20 | Tm_R_1 | 59.795 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 87071 | Primer End | 87286 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGCTGTTATCCGCTACATT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCAATAGCGGCATCCAAAAT | |||||||||||||
| Tm_F_2 | 60.117 | Size_F_2 | 20 | Tm_R_2 | 60.424 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 87071 | Primer End | 87316 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGCTGTTATCCGCTACATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTTCAATAGCGGCATCCAA | |||||||||||||
| Tm_F_3 | 60.117 | Size_F_3 | 20 | Tm_R_3 | 60.424 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 87071 | Primer End | 87319 |