Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)13
Start:73488 End:73500
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGGAAATGGAAATTGATGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGAACATCTTTCCCCTTG | |||||||||||||
| Tm_F_1 | 59.622 | Size_F_1 | 20 | Tm_R_1 | 59.665 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 73315 | Primer End | 73591 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGGAAATGGAAATTGATGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCATGAACCCCCTTTGTAT | |||||||||||||
| Tm_F_2 | 59.622 | Size_F_2 | 20 | Tm_R_2 | 60.94 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 73315 | Primer End | 73571 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGAAATGGAAATTGATGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCATGAACCCCCTTTGTATTT | |||||||||||||
| Tm_F_3 | 59.622 | Size_F_3 | 20 | Tm_R_3 | 59.932 | Size_R_3 | 21 | PRODUCT Size | 256 bp | Primer Start | 73315 | Primer End | 73570 |