Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)13
Start:25261 End:25273
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTTTGCCTCGACGGTTATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGGGGAATTGGTCTATGG | |||||||||||||
| Tm_F_1 | 60.132 | Size_F_1 | 20 | Tm_R_1 | 60.011 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 25101 | Primer End | 25337 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTTATGCCACGATGTCCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGGGGGAATTGGTCTATGG | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.011 | Size_R_2 | 20 | PRODUCT Size | 224 bp | Primer Start | 25114 | Primer End | 25337 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTTTGCCTCGACGGTTATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCCCAAATAAAAGGGGGAA | |||||||||||||
| Tm_F_3 | 60.132 | Size_F_3 | 20 | Tm_R_3 | 60.116 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 25101 | Primer End | 25348 |