Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)13
Start:133838 End:133850
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCCCAGGCCTCTGCTATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAGCTCAAAGGCGTAAAAC | |||||||||||||
| Tm_F_1 | 60.06 | Size_F_1 | 20 | Tm_R_1 | 60.018 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 133718 | Primer End | 133996 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCCAGGCCTCTGCTATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTGGATAGACTGGGCACC | |||||||||||||
| Tm_F_2 | 60.06 | Size_F_2 | 20 | Tm_R_2 | 59.933 | Size_R_2 | 20 | PRODUCT Size | 215 bp | Primer Start | 133718 | Primer End | 133932 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAAGGCTATCCCAGGCCTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTGGATAGACTGGGCACC | |||||||||||||
| Tm_F_3 | 60.187 | Size_F_3 | 20 | Tm_R_3 | 59.933 | Size_R_3 | 20 | PRODUCT Size | 222 bp | Primer Start | 133711 | Primer End | 133932 |