Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)12
Start:86097 End:86108
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTCATTCGCGGTAAAACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGTGGGATGGGGATTGTA | |||||||||||||
| Tm_F_1 | 59.943 | Size_F_1 | 20 | Tm_R_1 | 60.006 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 86019 | Primer End | 86258 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCATTCGCGGTAAAACCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAGGTGGGATGGGGATTGTA | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 60.006 | Size_R_2 | 20 | PRODUCT Size | 238 bp | Primer Start | 86021 | Primer End | 86258 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTCGCGGTAAAACCTCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCGGGGTATAATGACGGA | |||||||||||||
| Tm_F_3 | 59.966 | Size_F_3 | 20 | Tm_R_3 | 60.288 | Size_R_3 | 20 | PRODUCT Size | 203 bp | Primer Start | 86024 | Primer End | 86226 |