Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)12
Start:130063 End:130074
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAAATGAATCGCCCCTCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTGTGAGTGGAAAAATAGACCA | |||||||||||||
| Tm_F_1 | 60.399 | Size_F_1 | 20 | Tm_R_1 | 59.913 | Size_R_1 | 24 | PRODUCT Size | 245 bp | Primer Start | 130040 | Primer End | 130284 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGAAATAAGCGAGATGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTGTGAGTGGAAAAATAGACCA | |||||||||||||
| Tm_F_2 | 59.543 | Size_F_2 | 20 | Tm_R_2 | 59.913 | Size_R_2 | 24 | PRODUCT Size | 280 bp | Primer Start | 130005 | Primer End | 130284 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAAATGAATCGCCCCTCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGTGAGTGGAAAAATAGACCA | |||||||||||||
| Tm_F_3 | 60.399 | Size_F_3 | 20 | Tm_R_3 | 58.663 | Size_R_3 | 23 | PRODUCT Size | 244 bp | Primer Start | 130040 | Primer End | 130283 |