Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)10caatattgtgtttgttttaagatactatacaaacaatcgaagatatatatccaatccaaagagaaaggtaagtattta(T)10aggaaaggggattattaaataaaagaggattccagatacaagtacaaaaacgaatat(C)10
Start:30232 End:30396
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTGACTGAAAATACACAATCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTCGCCAAAACGGTACAAAA | |||||||||||||
| Tm_F_1 | 59.912 | Size_F_1 | 23 | Tm_R_1 | 60.895 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 30185 | Primer End | 30446 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGTGACTGAAAATACACAATCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGTCGCCAAAACGGTACA | |||||||||||||
| Tm_F_2 | 59.912 | Size_F_2 | 23 | Tm_R_2 | 59.98 | Size_R_2 | 19 | PRODUCT Size | 264 bp | Primer Start | 30185 | Primer End | 30448 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAATTTTTGCTTTGTGACTGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCGCCAAAACGGTACAAAA | |||||||||||||
| Tm_F_3 | 59.787 | Size_F_3 | 23 | Tm_R_3 | 60.895 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 30172 | Primer End | 30446 |