Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(T)10
Start:28524 End:28533
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATACCGGATCTCCGCCTAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGATATGGGCTCGTGTGAGT | |||||||||||||
| Tm_F_1 | 60.307 | Size_F_1 | 20 | Tm_R_1 | 59.957 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 28453 | Primer End | 28669 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATACCGGATCTCCGCCTAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGATATGGGCTCGTGTGAG | |||||||||||||
| Tm_F_2 | 60.307 | Size_F_2 | 20 | Tm_R_2 | 60.096 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 28453 | Primer End | 28670 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCCGCCTACACAAGCAAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATTGATCGAAAGGCAAGGA | |||||||||||||
| Tm_F_3 | 60.27 | Size_F_3 | 20 | Tm_R_3 | 60.155 | Size_R_3 | 20 | PRODUCT Size | 224 bp | Primer Start | 28463 | Primer End | 28686 |