Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(GCAAT)3
Start:105407 End:105421
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTAGTTAGCGATCCCGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTTGCTATCCTCTTGCCGA | |||||||||||||
| Tm_F_1 | 60.082 | Size_F_1 | 20 | Tm_R_1 | 59.978 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 105235 | Primer End | 105504 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGTGGACCGAGAAGAAAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTTGCTATCCTCTTGCCGA | |||||||||||||
| Tm_F_2 | 59.837 | Size_F_2 | 20 | Tm_R_2 | 59.978 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 105309 | Primer End | 105504 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTAGTTAGCGATCCCGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTGCTATCCTCTTGCCGAG | |||||||||||||
| Tm_F_3 | 60.082 | Size_F_3 | 20 | Tm_R_3 | 60.11 | Size_R_3 | 20 | PRODUCT Size | 269 bp | Primer Start | 105235 | Primer End | 105503 |