Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(C)13(A)10
Start:24789 End:24811
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATATCCCCCTTCAAAATCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTCATTGATTTTACGTCGCA | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 59.203 | Size_R_1 | 21 | PRODUCT Size | 279 bp | Primer Start | 24673 | Primer End | 24951 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTTTCATCCGGCTCAAGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTCATTGATTTTACGTCGCA | |||||||||||||
| Tm_F_2 | 60.206 | Size_F_2 | 20 | Tm_R_2 | 59.203 | Size_R_2 | 21 | PRODUCT Size | 247 bp | Primer Start | 24705 | Primer End | 24951 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGTTTCCTTTCATCCGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTCATTGATTTTACGTCGCA | |||||||||||||
| Tm_F_3 | 60.431 | Size_F_3 | 20 | Tm_R_3 | 59.203 | Size_R_3 | 21 | PRODUCT Size | 254 bp | Primer Start | 24698 | Primer End | 24951 |