Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(C)12
Start:4392 End:4403
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATGCTGCTTTTTCCATTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGATTTCGGTGTGCAATTA | |||||||||||||
| Tm_F_1 | 59.809 | Size_F_1 | 20 | Tm_R_1 | 59.93 | Size_R_1 | 20 | PRODUCT Size | 220 bp | Primer Start | 4243 | Primer End | 4462 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTGAACCGATCCGCTAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGATTTCGGTGTGCAATTA | |||||||||||||
| Tm_F_2 | 60.202 | Size_F_2 | 20 | Tm_R_2 | 59.93 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 4189 | Primer End | 4462 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTTTTGAACCGATCCGCTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGATTTCGGTGTGCAATTA | |||||||||||||
| Tm_F_3 | 59.708 | Size_F_3 | 20 | Tm_R_3 | 59.93 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 4187 | Primer End | 4462 |