Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(AT)6
Start:7395 End:7406
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGAGGATCCCCATACCTATCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTGCAGCCGGATTATTGAC | |||||||||||||
| Tm_F_1 | 60.049 | Size_F_1 | 23 | Tm_R_1 | 59.929 | Size_R_1 | 20 | PRODUCT Size | 265 bp | Primer Start | 7201 | Primer End | 7465 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAGAGGATCCCCATACCTATCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTACGCAGAAAGGAAGCGA | |||||||||||||
| Tm_F_2 | 60.049 | Size_F_2 | 23 | Tm_R_2 | 60.154 | Size_R_2 | 20 | PRODUCT Size | 231 bp | Primer Start | 7201 | Primer End | 7431 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGAGGATCCCCATACCTATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTGCAGCCGGATTATTGAC | |||||||||||||
| Tm_F_3 | 58.802 | Size_F_3 | 22 | Tm_R_3 | 59.929 | Size_R_3 | 20 | PRODUCT Size | 264 bp | Primer Start | 7202 | Primer End | 7465 |