Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(AAATAG)3
Start:1627 End:1644
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTCCCAAGCACACGATTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCGACTAGTTCCGGGTTC | |||||||||||||
| Tm_F_1 | 59.727 | Size_F_1 | 20 | Tm_R_1 | 60.328 | Size_R_1 | 20 | PRODUCT Size | 258 bp | Primer Start | 1602 | Primer End | 1859 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGCGGTCAATAAGGTAGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGATATCGGTTGACACGG | |||||||||||||
| Tm_F_2 | 59.953 | Size_F_2 | 20 | Tm_R_2 | 59.395 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 1431 | Primer End | 1708 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACGGTTTTCAGTGCTGGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGATATCGGTTGACACGG | |||||||||||||
| Tm_F_3 | 60.156 | Size_F_3 | 20 | Tm_R_3 | 59.395 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 1482 | Primer End | 1708 |