Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)15
Start:55113 End:55127
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTAGCCAAGCAGACAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTCCCCCGAATTAGTGAA | |||||||||||||
| Tm_F_1 | 60.022 | Size_F_1 | 20 | Tm_R_1 | 59.5 | Size_R_1 | 20 | PRODUCT Size | 197 bp | Primer Start | 54960 | Primer End | 55156 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAATCCCCAGGCTTTATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTCCCCCGAATTAGTGAA | |||||||||||||
| Tm_F_2 | 60.143 | Size_F_2 | 20 | Tm_R_2 | 59.5 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 54882 | Primer End | 55156 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGTAGTCGAAGCCCAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTTCCCCCGAATTAGTGAA | |||||||||||||
| Tm_F_3 | 60.241 | Size_F_3 | 20 | Tm_R_3 | 59.5 | Size_R_3 | 20 | PRODUCT Size | 239 bp | Primer Start | 54918 | Primer End | 55156 |