Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)15
Start:137291 End:137305
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCCACTGGATCTGTTCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGCGGAAAAATAGCTCGAC | |||||||||||||
| Tm_F_1 | 60.048 | Size_F_1 | 20 | Tm_R_1 | 59.982 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 137259 | Primer End | 137530 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCACTGGATCTGTTCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAAAATAGCTCGACGCCAG | |||||||||||||
| Tm_F_2 | 60.048 | Size_F_2 | 20 | Tm_R_2 | 59.982 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 137259 | Primer End | 137525 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGTCTCCACTGGATCTGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAAAATAGCTCGACGCCAG | |||||||||||||
| Tm_F_3 | 60.112 | Size_F_3 | 20 | Tm_R_3 | 59.982 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 137256 | Primer End | 137525 |