Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)12
Start:9644 End:9655
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCGGTACGAAAAACCCGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGATATCGCTTTCGAGCCCT | |||||||||||||
| Tm_F_1 | 59.99 | Size_F_1 | 20 | Tm_R_1 | 60.327 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 9436 | Primer End | 9714 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCGGTACGAAAAACCCGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCGCTTTCGAGCCCTTACT | |||||||||||||
| Tm_F_2 | 59.99 | Size_F_2 | 20 | Tm_R_2 | 60.36 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 9436 | Primer End | 9710 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCGGTACGAAAAACCCGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATCGCTTTCGAGCCCTTAC | |||||||||||||
| Tm_F_3 | 59.99 | Size_F_3 | 20 | Tm_R_3 | 59.456 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 9436 | Primer End | 9711 |