Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)12
Start:5081 End:5092
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCGCAATGCTTGGGTTTAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTCGTCTTCTATAACGACAAAAAT | |||||||||||||
| Tm_F_1 | 59.967 | Size_F_1 | 20 | Tm_R_1 | 58.404 | Size_R_1 | 25 | PRODUCT Size | 276 bp | Primer Start | 5045 | Primer End | 5320 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTATCGCAATGCTTGGGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCGTCTTCTATAACGACAAAAAT | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 58.404 | Size_R_2 | 25 | PRODUCT Size | 278 bp | Primer Start | 5043 | Primer End | 5320 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCAATGCTTGGGTTTACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCGTCTTCTATAACGACAAAAAT | |||||||||||||
| Tm_F_3 | 60.131 | Size_F_3 | 20 | Tm_R_3 | 58.404 | Size_R_3 | 25 | PRODUCT Size | 274 bp | Primer Start | 5047 | Primer End | 5320 |