Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)11tgattaatgattgttgttgtttcaagaacaatcataataaaggcaatttctattcctatcatac(AGAAT)3ccgagtgtcatttcttaattattttattctttctttttttttattccagtaaagtaaaaagtaaatggaat(A)11
Start:10056 End:10227
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGGTCTTTCGTTTTTGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAAAGGCGTATGCCATAACA | |||||||||||||
| Tm_F_1 | 59.587 | Size_F_1 | 20 | Tm_R_1 | 59.507 | Size_R_1 | 21 | PRODUCT Size | 279 bp | Primer Start | 9999 | Primer End | 10277 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGGTCTTTCGTTTTTGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAGGCGTATGCCATAACA | |||||||||||||
| Tm_F_2 | 59.587 | Size_F_2 | 20 | Tm_R_2 | 58.187 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 9999 | Primer End | 10276 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGGGTCTTTCGTTTTTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAAAGGCGTATGCCATAACA | |||||||||||||
| Tm_F_3 | 59.956 | Size_F_3 | 19 | Tm_R_3 | 59.507 | Size_R_3 | 21 | PRODUCT Size | 280 bp | Primer Start | 9998 | Primer End | 10277 |