Primers Available
Organism:Castanea mollissima chloroplast
Repeat:(A)10gaatcaatgtgtagattgcagccctcattc(T)11
Start:77225 End:77275
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTCGATAAAGTCGGTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAGGCCAAAACAAATCCTTC | |||||||||||||
| Tm_F_1 | 59.42 | Size_F_1 | 20 | Tm_R_1 | 59.546 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 77133 | Primer End | 77349 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCGCTATCAACCTCTTGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGGCCAAAACAAATCCTTC | |||||||||||||
| Tm_F_2 | 59.297 | Size_F_2 | 20 | Tm_R_2 | 59.546 | Size_R_2 | 20 | PRODUCT Size | 253 bp | Primer Start | 77097 | Primer End | 77349 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGCTCGATAAAGTCGGTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGGCCAAAACAAATCCTTC | |||||||||||||
| Tm_F_3 | 58.917 | Size_F_3 | 20 | Tm_R_3 | 59.546 | Size_R_3 | 20 | PRODUCT Size | 218 bp | Primer Start | 77132 | Primer End | 77349 |