Primers Available
Organism:Euphlyctis hexadactylus mitochondrion
Repeat:(TTAA)3
Start:17523 End:17534
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGATTTTAACGTGCCACCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATTTTTGGGAAGAGAGGGG | |||||||||||||
| Tm_F_1 | 59.996 | Size_F_1 | 20 | Tm_R_1 | 59.875 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 17303 | Primer End | 17569 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGAGGATGAGCCCCACTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCACCCCCTTCAAACTATCG | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.926 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 17435 | Primer End | 17667 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGAGGATGAGCCCCACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCACCCCCTTCAAACTATCG | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.926 | Size_R_3 | 20 | PRODUCT Size | 234 bp | Primer Start | 17434 | Primer End | 17667 |