Primers Available
Organism:Euphlyctis hexadactylus mitochondrion
Repeat:(TTAA)3
Start:17214 End:17225
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGATTTTTAACGTGCCACCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATTTTTGGGAAGAGAGGGG | |||||||||||||
| Tm_F_1 | 59.996 | Size_F_1 | 20 | Tm_R_1 | 59.875 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 16994 | Primer End | 17260 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGAGGATGAGCCCCACTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCACCCCCTTCAAACTATCG | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.926 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 17126 | Primer End | 17358 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGAGGATGAGCCCCACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCACCCCCTTCAAACTATCG | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.926 | Size_R_3 | 20 | PRODUCT Size | 234 bp | Primer Start | 17125 | Primer End | 17358 |