Primers Available
Organism:Euphlyctis hexadactylus mitochondrion
Repeat:(CAC)4
Start:3131 End:3142
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACCGAGGAGATGTCTGTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTATTTCATCGTTCCCTCGC | |||||||||||||
| Tm_F_1 | 59.82 | Size_F_1 | 20 | Tm_R_1 | 60.038 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 3060 | Primer End | 3238 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACCGAGGAGATGTCTGTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAGCCAAACCATGATGCAAA | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.073 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 3060 | Primer End | 3306 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACCGAGGAGATGTCTGTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATGCAAAAGGTACGAGGGA | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 60.074 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 3060 | Primer End | 3294 |