Primers Available
Organism:Hoplobatrachus tigerinus mitochondrion
Repeat:(AATTT)3
Start:16725 End:16739
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCTTAGCGAAATCGAACAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTGGTCTCATATGGGGAGA | |||||||||||||
| Tm_F_1 | 59.851 | Size_F_1 | 20 | Tm_R_1 | 60.034 | Size_R_1 | 20 | PRODUCT Size | 199 bp | Primer Start | 16682 | Primer End | 16880 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCTTAGCGAAATCGAACAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATCGTCAGAAACCCTGGAT | |||||||||||||
| Tm_F_2 | 59.851 | Size_F_2 | 20 | Tm_R_2 | 59.927 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 16682 | Primer End | 16958 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCTTAGCGAAATCGAACAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTCTCATATGGGGAGACGA | |||||||||||||
| Tm_F_3 | 59.851 | Size_F_3 | 20 | Tm_R_3 | 59.886 | Size_R_3 | 20 | PRODUCT Size | 196 bp | Primer Start | 16682 | Primer End | 16877 |