Primers Available
Organism:Ambystoma unisexual lineage LJJJ mitochondrion
Repeat:(G)11
Start:5148 End:5158
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCAGGATTTTACCCCACAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACGATTCAATTTCCGTCG | |||||||||||||
| Tm_F_1 | 60.192 | Size_F_1 | 20 | Tm_R_1 | 60.448 | Size_R_1 | 20 | PRODUCT Size | 203 bp | Primer Start | 4990 | Primer End | 5192 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACCTGCAGGATTTTACCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAACGATTCAATTTCCGTCG | |||||||||||||
| Tm_F_2 | 60.693 | Size_F_2 | 20 | Tm_R_2 | 60.448 | Size_R_2 | 20 | PRODUCT Size | 207 bp | Primer Start | 4986 | Primer End | 5192 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGCAGGATTTTACCCCACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAACGATTCAATTTCCGTCG | |||||||||||||
| Tm_F_3 | 60.883 | Size_F_3 | 20 | Tm_R_3 | 60.448 | Size_R_3 | 20 | PRODUCT Size | 204 bp | Primer Start | 4989 | Primer End | 5192 |