Primers Available
Organism:Ambystoma unisexual lineage LJJJ mitochondrion
Repeat:(ATTT)5
Start:7802 End:7821
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTCGGTGATTTCCAACCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATCATGGGACGGTTATTGC | |||||||||||||
| Tm_F_1 | 60.17 | Size_F_1 | 20 | Tm_R_1 | 59.651 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 7720 | Primer End | 7994 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTCGGTGATTTCCAACCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCATGGGACGGTTATTGCT | |||||||||||||
| Tm_F_2 | 60.17 | Size_F_2 | 20 | Tm_R_2 | 59.273 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 7720 | Primer End | 7993 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTCGGTGATTTCCAACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATGGGACGGTTATTGCTA | |||||||||||||
| Tm_F_3 | 60.17 | Size_F_3 | 20 | Tm_R_3 | 58.991 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 7720 | Primer End | 7992 |