Primers Available
Organism:Physemacris variolosa mitochondrion
Repeat:(AATA)3
Start:13696 End:13707
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTTAAGAGCTTATCCCCTAAAGAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTTTTGTATCAGGGTTTATTTAATTT | |||||||||||||
| Tm_F_1 | 58.619 | Size_F_1 | 26 | Tm_R_1 | 58.51 | Size_R_1 | 27 | PRODUCT Size | 250 bp | Primer Start | 13488 | Primer End | 13737 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAGCTTATCCCCTAAAGAATTAAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGTTTATAGTACCTTTTGTATCAGGG | |||||||||||||
| Tm_F_2 | 58.835 | Size_F_2 | 26 | Tm_R_2 | 58.261 | Size_R_2 | 27 | PRODUCT Size | 256 bp | Primer Start | 13494 | Primer End | 13749 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGCTTATCCCCTAAAGAATTAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTTTATAGTACCTTTTGTATCAGGG | |||||||||||||
| Tm_F_3 | 57.754 | Size_F_3 | 25 | Tm_R_3 | 58.261 | Size_R_3 | 27 | PRODUCT Size | 256 bp | Primer Start | 13494 | Primer End | 13749 |