Primers Available
Organism:Stichopus horrens mitochondrion
Repeat:(ATT)4
Start:6466 End:6477
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCTCTCTATCCTCGCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAGTAAGGCTACGGTGGAA | |||||||||||||
| Tm_F_1 | 60.059 | Size_F_1 | 20 | Tm_R_1 | 60.125 | Size_R_1 | 20 | PRODUCT Size | 166 bp | Primer Start | 6439 | Primer End | 6604 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAATCCGAAAAGCCAAAGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAGTAAGGCTACGGTGGAA | |||||||||||||
| Tm_F_2 | 60.181 | Size_F_2 | 20 | Tm_R_2 | 60.125 | Size_R_2 | 20 | PRODUCT Size | 213 bp | Primer Start | 6392 | Primer End | 6604 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGACCCTCTCTATCCTCGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAGTAAGGCTACGGTGGAA | |||||||||||||
| Tm_F_3 | 59.801 | Size_F_3 | 20 | Tm_R_3 | 60.125 | Size_R_3 | 20 | PRODUCT Size | 168 bp | Primer Start | 6437 | Primer End | 6604 |