Primers Available
Organism:Stichopus sp. SF-2010 mitochondrion
Repeat:(T)10
Start:11345 End:11354
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAAACGCTTGCTTGGTGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAGCGGTTAAGAAGGTGAG | |||||||||||||
| Tm_F_1 | 59.791 | Size_F_1 | 20 | Tm_R_1 | 60.008 | Size_R_1 | 20 | PRODUCT Size | 162 bp | Primer Start | 11280 | Primer End | 11441 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAAACGCTTGCTTGGTGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATATCTGCTGCCCCCAAAG | |||||||||||||
| Tm_F_2 | 59.791 | Size_F_2 | 20 | Tm_R_2 | 60.053 | Size_R_2 | 20 | PRODUCT Size | 259 bp | Primer Start | 11280 | Primer End | 11538 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAAACGCTTGCTTGGTGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTATATCTGCTGCCCCCAAA | |||||||||||||
| Tm_F_3 | 59.791 | Size_F_3 | 20 | Tm_R_3 | 60.053 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 11280 | Primer End | 11539 |