Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(TGAA)3
Start:6921 End:6932
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCGACGAAGAGAAATTGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGTTCGACCCGTCAGGTAT | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 59.989 | Size_R_1 | 20 | PRODUCT Size | 184 bp | Primer Start | 6781 | Primer End | 6964 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTGTCAACCGGTTTTTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGTTCGACCCGTCAGGTAT | |||||||||||||
| Tm_F_2 | 59.948 | Size_F_2 | 20 | Tm_R_2 | 59.989 | Size_R_2 | 20 | PRODUCT Size | 229 bp | Primer Start | 6736 | Primer End | 6964 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACCGGTTTTTCCCTTATCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTGTTCGACCCGTCAGGTAT | |||||||||||||
| Tm_F_3 | 60.177 | Size_F_3 | 20 | Tm_R_3 | 59.989 | Size_R_3 | 20 | PRODUCT Size | 222 bp | Primer Start | 6743 | Primer End | 6964 |