Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(T)10
Start:50603 End:50612
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTACGCTCCACTACACCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTTCGCCAAGCAGTTAAAG | |||||||||||||
| Tm_F_1 | 59.993 | Size_F_1 | 20 | Tm_R_1 | 59.883 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 50446 | Primer End | 50707 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTACGCTCCACTACACCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAGTGGCATGTTGGGTTC | |||||||||||||
| Tm_F_2 | 59.993 | Size_F_2 | 20 | Tm_R_2 | 59.836 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 50446 | Primer End | 50722 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTACGCTCCACTACACCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTGCATGACCGAGAATGTC | |||||||||||||
| Tm_F_3 | 59.993 | Size_F_3 | 20 | Tm_R_3 | 59.794 | Size_R_3 | 20 | PRODUCT Size | 221 bp | Primer Start | 50446 | Primer End | 50666 |