Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(AT)7
Start:8609 End:8622
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCACTCGAATTTACCCGAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGAGCTGCCAATAATGCAA | |||||||||||||
| Tm_F_1 | 59.931 | Size_F_1 | 20 | Tm_R_1 | 59.976 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 8545 | Primer End | 8763 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCACTCGAATTTACCCGAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACCAATGCCAAAACAGCAA | |||||||||||||
| Tm_F_2 | 59.931 | Size_F_2 | 20 | Tm_R_2 | 60.111 | Size_R_2 | 20 | PRODUCT Size | 177 bp | Primer Start | 8545 | Primer End | 8721 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCACTCGAATTTACCCGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTACTAGCGGTCCTGATGCC | |||||||||||||
| Tm_F_3 | 59.931 | Size_F_3 | 20 | Tm_R_3 | 60.235 | Size_R_3 | 20 | PRODUCT Size | 199 bp | Primer Start | 8545 | Primer End | 8743 |