Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(AAAC)3
Start:114521 End:114532
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATTTCGTGGCCGAGTATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTTACTACCCCAGCAACC | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 59.993 | Size_R_1 | 20 | PRODUCT Size | 218 bp | Primer Start | 114410 | Primer End | 114627 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAAATTTCGTGGCCGAGTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTTACTACCCCAGCAACC | |||||||||||||
| Tm_F_2 | 59.96 | Size_F_2 | 20 | Tm_R_2 | 59.993 | Size_R_2 | 20 | PRODUCT Size | 219 bp | Primer Start | 114409 | Primer End | 114627 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGGCCGAGTATGAACGTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGTTACTACCCCAGCAACC | |||||||||||||
| Tm_F_3 | 60.142 | Size_F_3 | 20 | Tm_R_3 | 59.993 | Size_R_3 | 20 | PRODUCT Size | 211 bp | Primer Start | 114417 | Primer End | 114627 |