Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(A)11
Start:66811 End:66821
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTACGTGCGGTGACATACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTTGAATCCGGAGTATT | |||||||||||||
| Tm_F_1 | 59.664 | Size_F_1 | 20 | Tm_R_1 | 60.017 | Size_R_1 | 20 | PRODUCT Size | 180 bp | Primer Start | 66742 | Primer End | 66921 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACACTACGTGCGGTGACAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGTTGAATCCGGAGTATT | |||||||||||||
| Tm_F_2 | 59.628 | Size_F_2 | 20 | Tm_R_2 | 60.017 | Size_R_2 | 20 | PRODUCT Size | 183 bp | Primer Start | 66739 | Primer End | 66921 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTGACACTACGTGCGGTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGTTGAATCCGGAGTATT | |||||||||||||
| Tm_F_3 | 60.381 | Size_F_3 | 20 | Tm_R_3 | 60.017 | Size_R_3 | 20 | PRODUCT Size | 186 bp | Primer Start | 66736 | Primer End | 66921 |