Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(A)11
Start:149422 End:149432
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTTACGCGTCTTTACCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACTCCCGAAATTACACGCC | |||||||||||||
| Tm_F_1 | 60.264 | Size_F_1 | 20 | Tm_R_1 | 59.96 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 149380 | Primer End | 149659 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGGGCTTACGCGTCTTTAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGACTCCTCCCAAGACAAAA | |||||||||||||
| Tm_F_2 | 59.904 | Size_F_2 | 20 | Tm_R_2 | 60.224 | Size_R_2 | 20 | PRODUCT Size | 264 bp | Primer Start | 149377 | Primer End | 149640 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGGGCTTACGCGTCTTTAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAGCCACGTGCTCTAATCCC | |||||||||||||
| Tm_F_3 | 59.904 | Size_F_3 | 20 | Tm_R_3 | 60.235 | Size_R_3 | 20 | PRODUCT Size | 153 bp | Primer Start | 149377 | Primer End | 149529 |