Primers Available
Organism:Pteridium aquilinum subsp. aquilinum chloroplast
Repeat:(A)10
Start:65041 End:65050
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAATGCCGGGTACTCAAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGGCCGTAGAAAGGTAAA | |||||||||||||
| Tm_F_1 | 60.856 | Size_F_1 | 20 | Tm_R_1 | 59.96 | Size_R_1 | 20 | PRODUCT Size | 184 bp | Primer Start | 64967 | Primer End | 65150 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAATGCCGGGTACTCAAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACGTGTTGCATCAGAATCC | |||||||||||||
| Tm_F_2 | 60.856 | Size_F_2 | 20 | Tm_R_2 | 60.12 | Size_R_2 | 20 | PRODUCT Size | 160 bp | Primer Start | 64967 | Primer End | 65126 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAATGCCGGGTACTCAAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACCAGCCAACAAACCAAAC | |||||||||||||
| Tm_F_3 | 60.856 | Size_F_3 | 20 | Tm_R_3 | 59.875 | Size_R_3 | 20 | PRODUCT Size | 221 bp | Primer Start | 64967 | Primer End | 65187 |