Primers Available
Organism:Phakopsora pachyrhizi mitochondrion
Repeat:(TGTATC)3
Start:25904 End:25921
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATACTGTCGATGGTGGGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTTTCCGAGACTCGAACT | |||||||||||||
| Tm_F_1 | 59.661 | Size_F_1 | 20 | Tm_R_1 | 60.756 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 25732 | Primer End | 26005 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGGTAAGCAGAGTATGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCTTTCCGAGACTCGAACT | |||||||||||||
| Tm_F_2 | 60.703 | Size_F_2 | 20 | Tm_R_2 | 60.756 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 25778 | Primer End | 26005 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTAAGCAGAGTATGGGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCTTTCCGAGACTCGAACT | |||||||||||||
| Tm_F_3 | 59.969 | Size_F_3 | 21 | Tm_R_3 | 60.756 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 25779 | Primer End | 26005 |