Primers Available
Organism:Phakopsora pachyrhizi mitochondrion
Repeat:(T)10
Start:11049 End:11058
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGGAGTAGAGCCACTGAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGAATCCCATGGAAACCTG | |||||||||||||
| Tm_F_1 | 59.308 | Size_F_1 | 20 | Tm_R_1 | 60.173 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 10816 | Primer End | 11081 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCGGGAGTAGAGCCACTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGAATCCCATGGAAACCTG | |||||||||||||
| Tm_F_2 | 60.268 | Size_F_2 | 19 | Tm_R_2 | 60.173 | Size_R_2 | 20 | PRODUCT Size | 268 bp | Primer Start | 10814 | Primer End | 11081 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTTACAATTTCGGATGGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATACAGTGCTGCTGCCTCAA | |||||||||||||
| Tm_F_3 | 58.622 | Size_F_3 | 20 | Tm_R_3 | 59.621 | Size_R_3 | 20 | PRODUCT Size | 169 bp | Primer Start | 11015 | Primer End | 11183 |