Primers Available
Organism:Phakopsora pachyrhizi mitochondrion
Repeat:(AGATA)11
Start:27593 End:27647
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTGACATTACTCCCTCCGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTAATGATGCACGATCCCTC | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 21 | Tm_R_1 | 59.915 | Size_R_1 | 21 | PRODUCT Size | 222 bp | Primer Start | 27456 | Primer End | 27677 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTGACATTACTCCCTCCGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGATGCACGATCCCTCATA | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 21 | Tm_R_2 | 58.903 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 27456 | Primer End | 27673 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCGATCGTAATCGGGATAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTAATGATGCACGATCCCTC | |||||||||||||
| Tm_F_3 | 58.882 | Size_F_3 | 20 | Tm_R_3 | 59.915 | Size_R_3 | 21 | PRODUCT Size | 271 bp | Primer Start | 27407 | Primer End | 27677 |