Primers Available
Organism:Setaria digitata mitochondrion
Repeat:(T)15
Start:12474 End:12488
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTTTTGTTTTTGCCTTTTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTACCACTAAAAACAATTAAACCA | |||||||||||||
| Tm_F_1 | 60 | Size_F_1 | 21 | Tm_R_1 | 57.787 | Size_R_1 | 26 | PRODUCT Size | 242 bp | Primer Start | 12296 | Primer End | 12537 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTTGGTGTTTCTCGTTTGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAACAGAAAACATAGATCCAGAACG | |||||||||||||
| Tm_F_2 | 57.832 | Size_F_2 | 22 | Tm_R_2 | 59.952 | Size_R_2 | 25 | PRODUCT Size | 194 bp | Primer Start | 12449 | Primer End | 12642 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTTGGTGTTTCTCGTTTGTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACAGAAAACATAGATCCAGAACG | |||||||||||||
| Tm_F_3 | 57.832 | Size_F_3 | 22 | Tm_R_3 | 58.761 | Size_R_3 | 24 | PRODUCT Size | 193 bp | Primer Start | 12449 | Primer End | 12641 |