Primers Available
Organism:Setaria digitata mitochondrion
Repeat:(T)13
Start:478 End:490
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTCCTTTTCATTTTTGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCACAACGAGCATATCAGAAAA | |||||||||||||
| Tm_F_1 | 60.188 | Size_F_1 | 20 | Tm_R_1 | 60.131 | Size_R_1 | 22 | PRODUCT Size | 209 bp | Primer Start | 357 | Primer End | 565 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCTCCTTTTCATTTTTGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCACAACGAGCATATCAGAAA | |||||||||||||
| Tm_F_2 | 60.188 | Size_F_2 | 20 | Tm_R_2 | 58.785 | Size_R_2 | 21 | PRODUCT Size | 209 bp | Primer Start | 357 | Primer End | 565 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCTCCTTTTCATTTTTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAACCACAACGAGCATATCA | |||||||||||||
| Tm_F_3 | 60.188 | Size_F_3 | 20 | Tm_R_3 | 58.639 | Size_R_3 | 21 | PRODUCT Size | 213 bp | Primer Start | 357 | Primer End | 569 |