Primers Available
Organism:Setaria digitata mitochondrion
Repeat:(T)10
Start:8515 End:8524
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGGCTTTGTTTGATGGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAACAAGAACGCATACCACC | |||||||||||||
| Tm_F_1 | 60.118 | Size_F_1 | 20 | Tm_R_1 | 59.05 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 8405 | Primer End | 8674 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGTTTGTGCCTGTTTGTGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAACAAGAACGCATACCACCT | |||||||||||||
| Tm_F_2 | 60.049 | Size_F_2 | 20 | Tm_R_2 | 60.045 | Size_R_2 | 21 | PRODUCT Size | 193 bp | Primer Start | 8482 | Primer End | 8674 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTGCAGGCTTTGTTTGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAACAAGAACGCATACCACC | |||||||||||||
| Tm_F_3 | 59.849 | Size_F_3 | 20 | Tm_R_3 | 59.05 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 8402 | Primer End | 8674 |